John lopez laboratory report #2: unknown bacteria no 11 abstract for the purpose of this exercise, the lists of possible unknown bacteria are: staphylococcus aureus, escherichia coli, salmonella typhimurium, enterobacter aerogenes, and enterococcus faecalis. - both e coli & enterobacter aerogenes are positive in all fermentations (lactose, glucose & mannitol) - both staph aureous & enterococcus faecalis are also positive in all fermentations - salmonella typhimurium is negative in fermentation of lactose, and positive in the others. 17 identification of unknown bacteria identify unknown bacteria review microbiology core technical competencies introduction identification of unknown bacteria will allow the student to review. This is a test commonly used when trying to identify gram-negative enteric bacteria, all of which are glucose fermenters but only some of which produce gas like msa, this medium also contains the ph indicator, phenol red enterobacter aerogenes and klebsiella pneumoniae are mr- and vp+ pseudomonas aeruginosa is a glucose nonfermenter and. The possible gram negative bacteria included: enterobacter aerogenes, pseudomonas aeruginosa, neisseria flavescens, proteus vulgaris, and moraxella catarrhalis the mixed culture tested in this report was #19.
The enterobacter aerogenes remained the same color, so it was urease negative (figure 14) the escherichia coli remained the same color, so it was also urease negative (figure 15) the proteus vulgaris turned red, meaning it became alkaline with the production of ammonia, so it was urease positive (figure 16. The lab instructor provided information that one species was a gram-positive bacteria and the other species was a gram-negative therefore, the next necessary step for correct identification of each unknown was to complete a gram staining procedure described on page 67 of the lab manual (2. Unknown lab report unknown organism #6 ann le (phuoc) may 6, 2010 dr carrington microbiology lab- mw 12:50 le 1 i introduction my unknown organism #6 is morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora (3, 5) this.
Enterobacter aerogenes is a gram negative rod shaped bacteria it is a nosocomial and a pathogenic bacterium that causes most of the opportunistic infections. Microbiology unknown lab report by cheryl a glaeser enterobacter aerogenes and enterococcus faecalis introduction: bacteria serve many different roles in people’s lives today it is important to identify bacteria and differentiate pathogenic and non-pathogenic organisms. Example of unknown lab report for microbiology procedures found in mcdonald et al (2011) the purpose of this test was to assist in confirming the identity of the unknown gram negative bacterium next, a urea test was performed on the gram negative bacterium enterobacter aerogenes | how to identify for micro unknown lab report post.
Lipopolysaccharide (lps) of gram negative bacteria, characteristics and functions april 29, 2013 tankeshwar microbiology for beginners , structure of bacterial cells 1 lipopolysaccharide (lps) layer also called the outer membrane is the outer most layer present in the cell wall of gram negative bacteria. Maura gongora 12th december, 2014 mcb 3020l- section 018 unknown #16 abstract this report contains the background information on gram positive and gram negative bacteria, which will aid in understanding the use of specific laboratory experiments to distinguish between the two types of bacteria. In this lab, students identify an unknown bacteria using a biochemical method and a molecular method for the biochemical method, students use a they pair up with a student with a gram negative unknown since the following methods in this lab were developed for gram enterobacter aerogenes negative negative 36361 instructor pages - - 8.
Gram negative unknown lab report enterobacter aerogenes name: danielle title: unknown lab report introduction: there are many reasons for identifying an unknown bacterium the reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. Unknown a had a positive result meaning that is was able to use citrate as a sole source of carbon, confirming that the unknown a was enterobacter aerogenes enterobacter aerogenes ( e aerogenes) is in the enterobacteriaceae family (2. For the gram stain, my organism turned red, meaning that my bacterium was a gram negative and it was also rod shaped after this test, the possible organisms were: enterobacter aerogenes, escherichia coli, serratia marcescens, citrobacter freundii, proteus mirabilis, pseudomonas aeruginosa, pseudomonas fluorescens, salmonella or shigella. Triple sugar iron agar triple sugar iron agar (tsi) is a differential medium that contains lactose, sucrose, a small amount of glucose (dextrose), ferrous sulfate, and the ph indicator phenol red. Microbiology unknown lab report - download as pdf file (pdf), text file (txt) or read online escherichia coli flowchart unknown g gram stain gram negative rod oxidase test (positive) positive citrobacter freundii enterobacter aerogenes escherichia coli klebsiella oxytoca klebsiella pneumoniae pseudomonas aeruginosa pseudomonas.
With negative results for this test, a variety of bacteria were able to be eliminated from the list of bacteria, including staphylococcus aureus, pseudomonas aeruginosa, and enterobacter aerogenes the emb test (figure 10), helped to further eliminate more bacteria including e coli. Mixed culture unknown identification report introduction: the objective of this experiment is to identify the organisms in a mixed culture of two unknown bacteria one organism is known to be a gram positive, and the other a gram negative it is important to isolate colonies of individual organisms. Start studying micro lab final- all unknown bacteria learn vocabulary, terms, and more with flashcards, games, and other study tools. On the next lab day, the unknowns were inoculated into a tube containing a thioglycollate stain, and the following were options for unknown #72: enterobacter aerogenes, escherichia coli, pseudomonas aeruginosa, pseudomonas fluorescens, proteus mirabilis, proteus vulgaris, after discovering that the unknown was gram negative, and examining.
Gram stain, differentiation bacterial growth media, cell morphology, colony streak isolation, and also record the various tests to turn in at the end of 3 weeks with the final result. Unknown lab report unknown organism #6 ann le (phuoc) may 6, 2010 dr carrington microbiology lab- mw 12:50 le 1 i introduction my unknown organism #6 is morganella morganii, which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans, mammals, and reptiles as a normal flora. Identification of an unknown bacterial species of enterobacteriaceae by means of biochemical characterization testing lab report #2 was indeed enterobacter aerogenes and many enzymes that work within introduction enterobacteriaceae are a very diverse group of organisms they are a gram-negative species that can live as a single cell, in. Unknown lab report: enterobacter cloacae jessica sainvil professor cupido bio 253l1 thu 9:30-12:30 due date: 12/6/12 unknown#103 introduction the bacterium enterobacter cloacae isolated from the given #103 was a gram-negative rod the purpose of this lab was to isolate and identify the genus and species of an unknown bacterium.
Unknown lab report april 25th, 2006 introduction the purpose of this lab was to identify two unknown bacteria cultures using various differential tests the identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Citrobacter diversus, c freundii, enterobacter aerogenes, e cloacae, erwinia chrysanthemi these tests are used to distinguish between members of the family enterobacteriacea and differentiate them from other gram-negative rods images from the lab manual the purpose of this paper is to demonstrate the ability to identify an unknown. The unknown bacteria will be one of the following: enterococcus faecalis, staphylococcus saprophyticus, escherichia coli, enterobacter aerogenes, proteus vulgaris, salmonella [i assume typhimurium], or shigella [either flexneri or sonnei, we used both in our lab during the semester.